PURPOSE: The purpose of the study was to investigate the effect of a 1 week creatine supplementation (CrS) on blood pressure (BP), mean arterial pressure (MAP), and pulse pressure (PP) in healthy males and females. METHODS: Subjects were 10 males (21±1.2 year) and 4 females (20.3 ± 0.47 year), with VO2max values of 53.53 ± 4.6 ml/kg/min (males) and 47.7 ± 2.9 ml/kg/min (females). Subjects were divided into two groups: placebo supplementation (PS) and CrS. Subjects attended a pre-supplementation and post-supplementation exercise intervention where subjects ran for 30 min at 70-80% of VO2max. Between the exercise interventions, subjects consumed 0.3 g/kg of body weight/day of supplement for 7 days. Following each exercise intervention, subjects wore an ambulatory blood pressure monitor (ABPM) for 24 hr to collect BP, MAP, and PP. RESULTS: A 7 day CrS will result in benefits to cardiovascular health (CVH). The CrS group will have lower mean BP, MAP, and PP when compared to the PS group following the second exercise intervention. CONCLUSIONS: The proposed research will contribute to greater understanding of the effects of CrS on CVH.